Kisspeptin-10 Technical Specification Sheet
Molecular Properties
| Parameter |
Value |
| Chemical Name |
Metastin (45-54) / Kisspeptin-10 |
| Amino Acid Sequence |
Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ |
| Molecular Formula |
C₆₃H₈₃N₁₇O₁₄ |
| Molecular Weight |
1302.45 g/mol |
| CAS Number |
374675-21-5 |
| Sequence Length |
10 amino acids (C-terminal decapeptide) |
| Receptor Target |
KISS1R (GPR54) |
Physical Properties
| Property |
Specification |
| Appearance |
White to off-white lyophilized powder |
| Solubility |
Soluble in sterile water, PBS (pH 7.0-7.4), or 0.9% saline at ≥1 mg/mL |
| pH (1% solution) |
5.5 - 7.0 |
| Hygroscopicity |
Moderate; store in desiccated environment |
| Solution Stability |
Stable at 4°C for 48-72 hours after reconstitution |
Analytical Specifications
| Test |
Specification |
| HPLC Purity |
≥98.0% |
| Mass Spectrometry |
Confirmed [M+H]⁺ = 1302.45 ± 0.5 Da |
| Peptide Content |
≥95% (by amino acid analysis) |
| Endotoxin Level |
<1.0 EU/mg |
| Acetate Content |
≤5% |
| TFA Content |
≤0.1% |
| Water Content |
≤8% (Karl Fischer) |
Storage Parameters
| Condition |
Specification |
| Lyophilized Storage |
-20°C to -80°C, desiccated |
| Lyophilized Shelf Life |
24-36 months when stored properly |
| Reconstituted Storage |
2-8°C for up to 14 days; -20°C for up to 3 months |
| Reconstitution Protocol |
Add 1.0 mL sterile water or PBS to 1 mg vial; gentle vortex 30-60 seconds |
| Freeze-Thaw Cycles |
Avoid multiple cycles; aliquot recommended |
| Light Sensitivity |
Protect from direct light; store in amber vials |
Research Dosing Reference
| Application |
Typical Dose Range |
Frequency |
Route |
| In Vitro Cell Studies |
10-1000 nM |
Single or repeated exposure |
Culture media |
| Small Animal Models (Rodent) |
0.1-10 nmol/animal (50-500 μg/kg) |
Once daily to BID |
IP, SC, IV |
| GnRH Stimulation Studies |
1-10 nmol/kg |
Acute or pulsatile |
IV bolus |
| Reproductive Axis Research |
0.5-5 nmol/kg |
Daily for 7-14 days |
SC injection |
Note: Dosing information is for research reference only. Kisspeptin-10 is intended strictly for in vitro and animal research purposes. Not approved for human use.
Key Research Studies
| Year |
Study Focus |
Key Findings |
| 2005 |
GPR54 receptor activation and GnRH secretion |
Demonstrated that kisspeptin-10 is the minimal active fragment capable of stimulating GnRH neurons and triggering LH release with EC₅₀ ~1 nM at GPR54 receptor |
| 2011 |
Hypothalamic-pituitary-gonadal axis regulation |
IV administration of kisspeptin-10 (0.01-1 nmol/kg) dose-dependently increased LH and FSH in male subjects; effects blocked by GnRH antagonists |
| 2015 |
Metabolic and reproductive integration |
Kisspeptin-10 infusion modulated both reproductive hormone secretion and metabolic parameters; demonstrated cross-talk between energy balance and fertility pathways |
| 2018 |
Structure-activity relationship analysis |
C-terminal Arg-Phe-NH₂ motif identified as critical for receptor binding; N-terminal modifications reduced potency by 10-100 fold compared to native kisspeptin-10 |
Mechanism of Action
| Biological Pathway |
Description |
| Receptor Binding |
High-affinity agonist at KISS1R (GPR54), a Gq-coupled GPCR expressed on GnRH neurons |
| Signal Transduction |
Activates phospholipase C → IP₃/DAG pathway → Ca²⁺ mobilization → neuronal depolarization |
| GnRH Release |
Direct stimulation of GnRH neurons in arcuate nucleus and AVPV; triggers GnRH pulsatile secretion |
| Downstream Effects |
LH and FSH release from pituitary gonadotrophs; subsequent gonadal steroidogenesis |
| Half-Life |
~30-40 minutes (IV); rapid degradation by peptidases |
Disclaimer: This product is intended for research use only. Not for human or veterinary diagnostic or therapeutic use. Handle according to institutional safety protocols.